The purpose of tThis study was to examine the effect of storage time and C-pPhycocyanin, an antioxidant substance purified from Arthrospira platensis, on spermatozoa in order to improve the storage of liquid ram semen storage. Sperm samples were collected from four adult fertile Sardi ramslambs and diluted to a final concentration of 800 million spermatozoa/ml in milk extender and C-pPhycocyanin at different concentrations. The samples were then stored at 15 °C and several sperm parameters (motility, viability, and morphology) were then evaluated at 0, 8, and 24 h. Results showed that the sperm the motility and kinematic parameters decreased significantly over time of storage, especially after 24 hours of storage, and that the C-pPhycocyanin used in this experiments had a significant eaffected significantly only at 1 and 2% concentrations at 0 h.,Eevaluation at 8 and 24 hours revealed that this substance didnotn’t have any positive effect on the storage of liquid ram semenstorage;, moreover, it had a negativelyeaffected, leading us to concludedoubt that the concentrations we used where not toxicpoisonous for spermatozoa.